Review



300 nm recombinant murine cxcl13  (R&D Systems)


Bioz Verified Symbol R&D Systems is a verified supplier
Bioz Manufacturer Symbol R&D Systems manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 90
      Buy from Supplier

    Structured Review

    R&D Systems 300 nm recombinant murine cxcl13
    Reconstitution of Immune Cell Functions following LV Gene Therapy in Primary Recipients Assays of immune cell function 16 weeks after BM transplantation. Bars show the mean ± SD. (A) The percentage of cells that underwent ≥1 cell division 72 hr after incubation with CD3/CD28 antibodies or P/I (as measured by CellTrace Violet dilution). Shown are cells within CD4 (left) or CD8 (right) staining gates by flow cytometry. Data are from one of the three independent experiments: n = 3 (KO and WT mock, MND.hWASp) and 5 (650.MND.hWASp). (B) Flow cytometry analysis showing CellTrace Violet labeled splenocytes 72 hr post-CD3/CD28 stimulation, gated on live and either CD4 + or CD8 + populations. Numbers indicate the percentages that have proliferated after CellTrace Violet labeling. (C) B cell (CD43 − splenocyte) migration in response to media only ( − ) or media supplemented with <t>CXCL13</t> chemokine ( + ). Each dot indicates the percentage of B cells from a single mouse that migrated through the 5-μm-pore transwell. Data are from two independent experiments: n = 5 (KO mock, 650.MND.hWASp), 3 (MND.hWASp), and 8 (WT mock). (D) IgG and IgM and (E) anti-double-stranded DNA antibody levels in the serum of primary recipients as determined by ELISA 16 weeks post-transplant. Data are from three independent experiments: n = 5 (KO and WT mock), 4 (MND.hWASp), 2 (WT) (D only), and 12 (650.MND.hWASp). Serum from an autoimmune WAS chimera (E only) with high serum anti-DNA antibodies was used as a positive control. The p value was determined by unpaired two-tailed t test. *p < 0.024.
    300 Nm Recombinant Murine Cxcl13, supplied by R&D Systems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/300 nm recombinant murine cxcl13/product/R&D Systems
    Average 90 stars, based on 1 article reviews
    300 nm recombinant murine cxcl13 - by Bioz Stars, 2026-03
    90/100 stars

    Images

    1) Product Images from "Safe and Effective Gene Therapy for Murine Wiskott-Aldrich Syndrome Using an Insulated Lentiviral Vector"

    Article Title: Safe and Effective Gene Therapy for Murine Wiskott-Aldrich Syndrome Using an Insulated Lentiviral Vector

    Journal: Molecular Therapy. Methods & Clinical Development

    doi: 10.1016/j.omtm.2016.11.001

    Reconstitution of Immune Cell Functions following LV Gene Therapy in Primary Recipients Assays of immune cell function 16 weeks after BM transplantation. Bars show the mean ± SD. (A) The percentage of cells that underwent ≥1 cell division 72 hr after incubation with CD3/CD28 antibodies or P/I (as measured by CellTrace Violet dilution). Shown are cells within CD4 (left) or CD8 (right) staining gates by flow cytometry. Data are from one of the three independent experiments: n = 3 (KO and WT mock, MND.hWASp) and 5 (650.MND.hWASp). (B) Flow cytometry analysis showing CellTrace Violet labeled splenocytes 72 hr post-CD3/CD28 stimulation, gated on live and either CD4 + or CD8 + populations. Numbers indicate the percentages that have proliferated after CellTrace Violet labeling. (C) B cell (CD43 − splenocyte) migration in response to media only ( − ) or media supplemented with CXCL13 chemokine ( + ). Each dot indicates the percentage of B cells from a single mouse that migrated through the 5-μm-pore transwell. Data are from two independent experiments: n = 5 (KO mock, 650.MND.hWASp), 3 (MND.hWASp), and 8 (WT mock). (D) IgG and IgM and (E) anti-double-stranded DNA antibody levels in the serum of primary recipients as determined by ELISA 16 weeks post-transplant. Data are from three independent experiments: n = 5 (KO and WT mock), 4 (MND.hWASp), 2 (WT) (D only), and 12 (650.MND.hWASp). Serum from an autoimmune WAS chimera (E only) with high serum anti-DNA antibodies was used as a positive control. The p value was determined by unpaired two-tailed t test. *p < 0.024.
    Figure Legend Snippet: Reconstitution of Immune Cell Functions following LV Gene Therapy in Primary Recipients Assays of immune cell function 16 weeks after BM transplantation. Bars show the mean ± SD. (A) The percentage of cells that underwent ≥1 cell division 72 hr after incubation with CD3/CD28 antibodies or P/I (as measured by CellTrace Violet dilution). Shown are cells within CD4 (left) or CD8 (right) staining gates by flow cytometry. Data are from one of the three independent experiments: n = 3 (KO and WT mock, MND.hWASp) and 5 (650.MND.hWASp). (B) Flow cytometry analysis showing CellTrace Violet labeled splenocytes 72 hr post-CD3/CD28 stimulation, gated on live and either CD4 + or CD8 + populations. Numbers indicate the percentages that have proliferated after CellTrace Violet labeling. (C) B cell (CD43 − splenocyte) migration in response to media only ( − ) or media supplemented with CXCL13 chemokine ( + ). Each dot indicates the percentage of B cells from a single mouse that migrated through the 5-μm-pore transwell. Data are from two independent experiments: n = 5 (KO mock, 650.MND.hWASp), 3 (MND.hWASp), and 8 (WT mock). (D) IgG and IgM and (E) anti-double-stranded DNA antibody levels in the serum of primary recipients as determined by ELISA 16 weeks post-transplant. Data are from three independent experiments: n = 5 (KO and WT mock), 4 (MND.hWASp), 2 (WT) (D only), and 12 (650.MND.hWASp). Serum from an autoimmune WAS chimera (E only) with high serum anti-DNA antibodies was used as a positive control. The p value was determined by unpaired two-tailed t test. *p < 0.024.

    Techniques Used: Cell Function Assay, Transplantation Assay, Incubation, Staining, Flow Cytometry, Labeling, Migration, Enzyme-linked Immunosorbent Assay, Positive Control, Two Tailed Test



    Similar Products

    300 nm recombinant murine cxcl13  (R&D Systems)
    90
    R&D Systems 300 nm recombinant murine cxcl13
    Reconstitution of Immune Cell Functions following LV Gene Therapy in Primary Recipients Assays of immune cell function 16 weeks after BM transplantation. Bars show the mean ± SD. (A) The percentage of cells that underwent ≥1 cell division 72 hr after incubation with CD3/CD28 antibodies or P/I (as measured by CellTrace Violet dilution). Shown are cells within CD4 (left) or CD8 (right) staining gates by flow cytometry. Data are from one of the three independent experiments: n = 3 (KO and WT mock, MND.hWASp) and 5 (650.MND.hWASp). (B) Flow cytometry analysis showing CellTrace Violet labeled splenocytes 72 hr post-CD3/CD28 stimulation, gated on live and either CD4 + or CD8 + populations. Numbers indicate the percentages that have proliferated after CellTrace Violet labeling. (C) B cell (CD43 − splenocyte) migration in response to media only ( − ) or media supplemented with <t>CXCL13</t> chemokine ( + ). Each dot indicates the percentage of B cells from a single mouse that migrated through the 5-μm-pore transwell. Data are from two independent experiments: n = 5 (KO mock, 650.MND.hWASp), 3 (MND.hWASp), and 8 (WT mock). (D) IgG and IgM and (E) anti-double-stranded DNA antibody levels in the serum of primary recipients as determined by ELISA 16 weeks post-transplant. Data are from three independent experiments: n = 5 (KO and WT mock), 4 (MND.hWASp), 2 (WT) (D only), and 12 (650.MND.hWASp). Serum from an autoimmune WAS chimera (E only) with high serum anti-DNA antibodies was used as a positive control. The p value was determined by unpaired two-tailed t test. *p < 0.024.
    300 Nm Recombinant Murine Cxcl13, supplied by R&D Systems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/300 nm recombinant murine cxcl13/product/R&D Systems
    Average 90 stars, based on 1 article reviews
    300 nm recombinant murine cxcl13 - by Bioz Stars, 2026-03
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    Reconstitution of Immune Cell Functions following LV Gene Therapy in Primary Recipients Assays of immune cell function 16 weeks after BM transplantation. Bars show the mean ± SD. (A) The percentage of cells that underwent ≥1 cell division 72 hr after incubation with CD3/CD28 antibodies or P/I (as measured by CellTrace Violet dilution). Shown are cells within CD4 (left) or CD8 (right) staining gates by flow cytometry. Data are from one of the three independent experiments: n = 3 (KO and WT mock, MND.hWASp) and 5 (650.MND.hWASp). (B) Flow cytometry analysis showing CellTrace Violet labeled splenocytes 72 hr post-CD3/CD28 stimulation, gated on live and either CD4 + or CD8 + populations. Numbers indicate the percentages that have proliferated after CellTrace Violet labeling. (C) B cell (CD43 − splenocyte) migration in response to media only ( − ) or media supplemented with CXCL13 chemokine ( + ). Each dot indicates the percentage of B cells from a single mouse that migrated through the 5-μm-pore transwell. Data are from two independent experiments: n = 5 (KO mock, 650.MND.hWASp), 3 (MND.hWASp), and 8 (WT mock). (D) IgG and IgM and (E) anti-double-stranded DNA antibody levels in the serum of primary recipients as determined by ELISA 16 weeks post-transplant. Data are from three independent experiments: n = 5 (KO and WT mock), 4 (MND.hWASp), 2 (WT) (D only), and 12 (650.MND.hWASp). Serum from an autoimmune WAS chimera (E only) with high serum anti-DNA antibodies was used as a positive control. The p value was determined by unpaired two-tailed t test. *p < 0.024.

    Journal: Molecular Therapy. Methods & Clinical Development

    Article Title: Safe and Effective Gene Therapy for Murine Wiskott-Aldrich Syndrome Using an Insulated Lentiviral Vector

    doi: 10.1016/j.omtm.2016.11.001

    Figure Lengend Snippet: Reconstitution of Immune Cell Functions following LV Gene Therapy in Primary Recipients Assays of immune cell function 16 weeks after BM transplantation. Bars show the mean ± SD. (A) The percentage of cells that underwent ≥1 cell division 72 hr after incubation with CD3/CD28 antibodies or P/I (as measured by CellTrace Violet dilution). Shown are cells within CD4 (left) or CD8 (right) staining gates by flow cytometry. Data are from one of the three independent experiments: n = 3 (KO and WT mock, MND.hWASp) and 5 (650.MND.hWASp). (B) Flow cytometry analysis showing CellTrace Violet labeled splenocytes 72 hr post-CD3/CD28 stimulation, gated on live and either CD4 + or CD8 + populations. Numbers indicate the percentages that have proliferated after CellTrace Violet labeling. (C) B cell (CD43 − splenocyte) migration in response to media only ( − ) or media supplemented with CXCL13 chemokine ( + ). Each dot indicates the percentage of B cells from a single mouse that migrated through the 5-μm-pore transwell. Data are from two independent experiments: n = 5 (KO mock, 650.MND.hWASp), 3 (MND.hWASp), and 8 (WT mock). (D) IgG and IgM and (E) anti-double-stranded DNA antibody levels in the serum of primary recipients as determined by ELISA 16 weeks post-transplant. Data are from three independent experiments: n = 5 (KO and WT mock), 4 (MND.hWASp), 2 (WT) (D only), and 12 (650.MND.hWASp). Serum from an autoimmune WAS chimera (E only) with high serum anti-DNA antibodies was used as a positive control. The p value was determined by unpaired two-tailed t test. *p < 0.024.

    Article Snippet: The lower wells had 600 μL lymphocyte media and 300 nM recombinant murine CXCL13 (R&D Systems).

    Techniques: Cell Function Assay, Transplantation Assay, Incubation, Staining, Flow Cytometry, Labeling, Migration, Enzyme-linked Immunosorbent Assay, Positive Control, Two Tailed Test